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g418  (Thermo Fisher)


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    Structured Review

    Thermo Fisher g418
    G418, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/g418+sulfate/G418+sulfate%2C+for+biochemistry/10__1158_slash_2326___6066__cir___25___1420-66-48-49
    Average 95 stars, based on 1 article reviews
    g418 - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Modification:

    Article Title: Comparison of Receptor‐Mediated Endocytosis and Its Application to Enhance DNA Transfection by TFAMoplex
    Article Snippet: Bovine serum albumin (BSA), glucose, Hoechst 33 342, kanamycin sulfate, methanol free paraformaldehyde, Laemmli buffer components (sodium dodecyl sulfate (SDS), glycerol, 2-mercaptoethanol, bromophenol blue, Tris-HCl), and poly(ethylenimine) (PEI, branched, 25 kDa, CAS 9002-98−6) were all purchased from Sigma–Aldrich (St. Louis, MO, USA). .. CellMask Deep Red, Dulbecco’s Modified Eagle Medium (DMEM) high glucose GlutaMAX, FBS, FreeStyle 293 Expression Medium, G418 sulfate, trypsin-ethylenediaminetetraacetic acid (EDTA) (0.05% w/v), 3M potassium chloride (KCl), Halt protease inhibitor cocktail, Gibco phosphate-buffered saline (PBS) (pH 7.4), pcDNA3.4 plasmid backbone, and Phusion DNA polymerase were obtained from Thermo Fisher Scientific (Waltham, MA, USA). .. Cholera toxin subunit-B CF647 and transferrin-CF488 were from Biotium (Fremont, CA, USA).

    Article Title: An Attenuated Recombinant Newcastle Disease Virus of Genotype VII Generated by Reverse Genetics
    Article Snippet: .. The cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Carlsbad, CA, USA) supplemented with 10% fetal calf serum and 1 mg/mL G418 Sulfate (Gibco, Carlsbad, CA, USA). ..

    Expressing:

    Article Title: Comparison of Receptor‐Mediated Endocytosis and Its Application to Enhance DNA Transfection by TFAMoplex
    Article Snippet: Bovine serum albumin (BSA), glucose, Hoechst 33 342, kanamycin sulfate, methanol free paraformaldehyde, Laemmli buffer components (sodium dodecyl sulfate (SDS), glycerol, 2-mercaptoethanol, bromophenol blue, Tris-HCl), and poly(ethylenimine) (PEI, branched, 25 kDa, CAS 9002-98−6) were all purchased from Sigma–Aldrich (St. Louis, MO, USA). .. CellMask Deep Red, Dulbecco’s Modified Eagle Medium (DMEM) high glucose GlutaMAX, FBS, FreeStyle 293 Expression Medium, G418 sulfate, trypsin-ethylenediaminetetraacetic acid (EDTA) (0.05% w/v), 3M potassium chloride (KCl), Halt protease inhibitor cocktail, Gibco phosphate-buffered saline (PBS) (pH 7.4), pcDNA3.4 plasmid backbone, and Phusion DNA polymerase were obtained from Thermo Fisher Scientific (Waltham, MA, USA). .. Cholera toxin subunit-B CF647 and transferrin-CF488 were from Biotium (Fremont, CA, USA).

    Article Title: Peripheral lysosome levels dictate mTORC1 inactivation even when catabolically impaired
    Article Snippet: .. HEK293A cells stably expressing dnATG4 were generated by transfection with pmStrawberry-Atg4B C74A , followed by selection with medium containing 400 μg/mL G418 sulfate (Thermo Fisher Scientific). ..

    Article Title: Peripheral lysosome levels dictate mTORC1 inactivation even when catabolically impaired.
    Article Snippet: .. HEK293A cells stably expressing dnATG4 were generated by transfection with pmStrawberry-Atg4BC74A, followed by selection with medium containing 400 μg/mL G418 sulfate (Thermo Fisher Scientific). .. Single colonies of expressing cells were then isolated and expanded. siRNAs and plasmids We used the following siRNAs from Sigma-Aldrich: Rap1A#1 (SASI_Hs1_00040405), Rap1A#2 (SASI_ Hs2_00305929), Rap1B#1 (SASI_Hs2_00305940), Rap1B#2 (SASI_Hs2_00305936), TSC2 (SASI_ Hs02_00312104) and universal negative control siRNA (SIC001).

    Protease Inhibitor:

    Article Title: Comparison of Receptor‐Mediated Endocytosis and Its Application to Enhance DNA Transfection by TFAMoplex
    Article Snippet: Bovine serum albumin (BSA), glucose, Hoechst 33 342, kanamycin sulfate, methanol free paraformaldehyde, Laemmli buffer components (sodium dodecyl sulfate (SDS), glycerol, 2-mercaptoethanol, bromophenol blue, Tris-HCl), and poly(ethylenimine) (PEI, branched, 25 kDa, CAS 9002-98−6) were all purchased from Sigma–Aldrich (St. Louis, MO, USA). .. CellMask Deep Red, Dulbecco’s Modified Eagle Medium (DMEM) high glucose GlutaMAX, FBS, FreeStyle 293 Expression Medium, G418 sulfate, trypsin-ethylenediaminetetraacetic acid (EDTA) (0.05% w/v), 3M potassium chloride (KCl), Halt protease inhibitor cocktail, Gibco phosphate-buffered saline (PBS) (pH 7.4), pcDNA3.4 plasmid backbone, and Phusion DNA polymerase were obtained from Thermo Fisher Scientific (Waltham, MA, USA). .. Cholera toxin subunit-B CF647 and transferrin-CF488 were from Biotium (Fremont, CA, USA).

    Saline:

    Article Title: Comparison of Receptor‐Mediated Endocytosis and Its Application to Enhance DNA Transfection by TFAMoplex
    Article Snippet: Bovine serum albumin (BSA), glucose, Hoechst 33 342, kanamycin sulfate, methanol free paraformaldehyde, Laemmli buffer components (sodium dodecyl sulfate (SDS), glycerol, 2-mercaptoethanol, bromophenol blue, Tris-HCl), and poly(ethylenimine) (PEI, branched, 25 kDa, CAS 9002-98−6) were all purchased from Sigma–Aldrich (St. Louis, MO, USA). .. CellMask Deep Red, Dulbecco’s Modified Eagle Medium (DMEM) high glucose GlutaMAX, FBS, FreeStyle 293 Expression Medium, G418 sulfate, trypsin-ethylenediaminetetraacetic acid (EDTA) (0.05% w/v), 3M potassium chloride (KCl), Halt protease inhibitor cocktail, Gibco phosphate-buffered saline (PBS) (pH 7.4), pcDNA3.4 plasmid backbone, and Phusion DNA polymerase were obtained from Thermo Fisher Scientific (Waltham, MA, USA). .. Cholera toxin subunit-B CF647 and transferrin-CF488 were from Biotium (Fremont, CA, USA).

    Plasmid Preparation:

    Article Title: Comparison of Receptor‐Mediated Endocytosis and Its Application to Enhance DNA Transfection by TFAMoplex
    Article Snippet: Bovine serum albumin (BSA), glucose, Hoechst 33 342, kanamycin sulfate, methanol free paraformaldehyde, Laemmli buffer components (sodium dodecyl sulfate (SDS), glycerol, 2-mercaptoethanol, bromophenol blue, Tris-HCl), and poly(ethylenimine) (PEI, branched, 25 kDa, CAS 9002-98−6) were all purchased from Sigma–Aldrich (St. Louis, MO, USA). .. CellMask Deep Red, Dulbecco’s Modified Eagle Medium (DMEM) high glucose GlutaMAX, FBS, FreeStyle 293 Expression Medium, G418 sulfate, trypsin-ethylenediaminetetraacetic acid (EDTA) (0.05% w/v), 3M potassium chloride (KCl), Halt protease inhibitor cocktail, Gibco phosphate-buffered saline (PBS) (pH 7.4), pcDNA3.4 plasmid backbone, and Phusion DNA polymerase were obtained from Thermo Fisher Scientific (Waltham, MA, USA). .. Cholera toxin subunit-B CF647 and transferrin-CF488 were from Biotium (Fremont, CA, USA).

    Transfection:

    Article Title: Impairment of oxidative metabolism compromises Rad51 recruitment and potentiates PARP inhibitor effectiveness in ovarian cancer.
    Article Snippet: Transfection with K200E-Fen1 1x106 shPGC-1β ID8 #47 cells were transfected with 4 μg pCMV6-K200EFen1 (Origene), encoding for a mutant form of Fen1 (point mutation c.598A>G) using the Gene Pulser II electroporator (Bio-Rad). .. Transfected cells were selected 48 h later and further expanded in their culture medium supplemented with using 1 mg/ml G418 Sulfate (Gibco). ..

    Article Title: Peripheral lysosome levels dictate mTORC1 inactivation even when catabolically impaired
    Article Snippet: .. HEK293A cells stably expressing dnATG4 were generated by transfection with pmStrawberry-Atg4B C74A , followed by selection with medium containing 400 μg/mL G418 sulfate (Thermo Fisher Scientific). ..

    Article Title: Peripheral lysosome levels dictate mTORC1 inactivation even when catabolically impaired.
    Article Snippet: .. HEK293A cells stably expressing dnATG4 were generated by transfection with pmStrawberry-Atg4BC74A, followed by selection with medium containing 400 μg/mL G418 sulfate (Thermo Fisher Scientific). .. Single colonies of expressing cells were then isolated and expanded. siRNAs and plasmids We used the following siRNAs from Sigma-Aldrich: Rap1A#1 (SASI_Hs1_00040405), Rap1A#2 (SASI_ Hs2_00305929), Rap1B#1 (SASI_Hs2_00305940), Rap1B#2 (SASI_Hs2_00305936), TSC2 (SASI_ Hs02_00312104) and universal negative control siRNA (SIC001).

    Article Title: Impairment of oxidative metabolism compromises Rad51 recruitment and potentiates PARP inhibitor effectiveness in ovarian cancer
    Article Snippet: 1 × 10 6 shPGC-1β ID8 #47 cells were transfected with 4 μg pCMV6-K200E-Fen1 (Origene), encoding for a mutant form of Fen1 (point mutation c.598 A > G) using the Gene Pulser II electroporator (Bio-Rad). .. Transfected cells were selected 48 h later and further expanded in their culture medium supplemented with using 1 mg/ml G418 Sulfate (Gibco). ..

    Stable Transfection:

    Article Title: Peripheral lysosome levels dictate mTORC1 inactivation even when catabolically impaired
    Article Snippet: .. HEK293A cells stably expressing dnATG4 were generated by transfection with pmStrawberry-Atg4B C74A , followed by selection with medium containing 400 μg/mL G418 sulfate (Thermo Fisher Scientific). ..

    Article Title: Peripheral lysosome levels dictate mTORC1 inactivation even when catabolically impaired.
    Article Snippet: .. HEK293A cells stably expressing dnATG4 were generated by transfection with pmStrawberry-Atg4BC74A, followed by selection with medium containing 400 μg/mL G418 sulfate (Thermo Fisher Scientific). .. Single colonies of expressing cells were then isolated and expanded. siRNAs and plasmids We used the following siRNAs from Sigma-Aldrich: Rap1A#1 (SASI_Hs1_00040405), Rap1A#2 (SASI_ Hs2_00305929), Rap1B#1 (SASI_Hs2_00305940), Rap1B#2 (SASI_Hs2_00305936), TSC2 (SASI_ Hs02_00312104) and universal negative control siRNA (SIC001).

    Generated:

    Article Title: Peripheral lysosome levels dictate mTORC1 inactivation even when catabolically impaired
    Article Snippet: .. HEK293A cells stably expressing dnATG4 were generated by transfection with pmStrawberry-Atg4B C74A , followed by selection with medium containing 400 μg/mL G418 sulfate (Thermo Fisher Scientific). ..

    Article Title: Peripheral lysosome levels dictate mTORC1 inactivation even when catabolically impaired.
    Article Snippet: .. HEK293A cells stably expressing dnATG4 were generated by transfection with pmStrawberry-Atg4BC74A, followed by selection with medium containing 400 μg/mL G418 sulfate (Thermo Fisher Scientific). .. Single colonies of expressing cells were then isolated and expanded. siRNAs and plasmids We used the following siRNAs from Sigma-Aldrich: Rap1A#1 (SASI_Hs1_00040405), Rap1A#2 (SASI_ Hs2_00305929), Rap1B#1 (SASI_Hs2_00305940), Rap1B#2 (SASI_Hs2_00305936), TSC2 (SASI_ Hs02_00312104) and universal negative control siRNA (SIC001).

    Selection:

    Article Title: Peripheral lysosome levels dictate mTORC1 inactivation even when catabolically impaired
    Article Snippet: .. HEK293A cells stably expressing dnATG4 were generated by transfection with pmStrawberry-Atg4B C74A , followed by selection with medium containing 400 μg/mL G418 sulfate (Thermo Fisher Scientific). ..

    Article Title: Peripheral lysosome levels dictate mTORC1 inactivation even when catabolically impaired.
    Article Snippet: .. HEK293A cells stably expressing dnATG4 were generated by transfection with pmStrawberry-Atg4BC74A, followed by selection with medium containing 400 μg/mL G418 sulfate (Thermo Fisher Scientific). .. Single colonies of expressing cells were then isolated and expanded. siRNAs and plasmids We used the following siRNAs from Sigma-Aldrich: Rap1A#1 (SASI_Hs1_00040405), Rap1A#2 (SASI_ Hs2_00305929), Rap1B#1 (SASI_Hs2_00305940), Rap1B#2 (SASI_Hs2_00305936), TSC2 (SASI_ Hs02_00312104) and universal negative control siRNA (SIC001).

    other:

    Article Title: A live-cell autophagy reporter reveals reversible vacuolation in naked mole-rat skin fibroblasts under lysosomal stress
    Article Snippet: HeLa Kyoto cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) + GlutaMAXTM (Gibco) with 10% Fetal Bovine Serum.



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